Name _____________________

Class _____________________

Dilution Worksheet

 

A. Calculate the final dilution of each of these series. Write your answer in scientific notation.

1. 1:1000, 1: 100, 1:100 ____________________

2. 1:10, 1:100, 1:1000 _____________________

3. 1/10, 1/1000, 1/100 _____________________

4. 10^-3, 10^-2, 10^-1 _____________________

5. 10^-1, 10^-3, 10^-3 _____________________

6. 1/100, 1/100, 1/100 _____________________

7. 1:100, 1:1000, 1:10,000 _____________________

8. 10^-2, 10^-2, 10^-1 _____________________

9. 1/1000, 1/1000, 1/10 _____________________

10. 10^-1, 10^-2, 10^-1 _____________________

 

B. Calculate the actual cell concentration (the number of cells per milliliter of solution) in the bacterial culture based on the plate counts and dilutions given below.

Plate counts (number of cells per plate counted) Final dilution (the amount that the original sample was diluted before being grown on the plate) Actual cells per milliliter in the original culture
202 10^-5

 

157 1:10,000  
180 1/1000  
106 10^-6  
85 1:100,000  
275 1/10,000  
200 10^-3  
99 10^-8  
155 1/10,000  
198 1:1,000,000